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Down-regulation of the stathmin/Op18 and FKBP25 genes following p53 induction

Oncogene volume 18, pages 5954–5958 (1999)Cite this article

Abstract

The p53 tumor suppressor protein can function as an activator and a repressor of gene transcription. Currently, the mechanism of transcriptional repression by p53 is poorly understood. To aid in clarifying this mechanism, we carried out studies designed to identify specific target genes that are down-regulated following p53 induction. Among the negative p53-response genes revealed by our screening protocols are those encoding stathmin (Op18), a tubulin-associated protein implicated in cell signaling pathways, and an FK506/rapamycin-binding protein, FKBP25. Stathmin and FKBP25 exhibit decreased expression in both human and murine immortalized and transformed cell lines following induction of wild-type p53 by several stimuli that result in DNA damage. Candidate p53-repressed genes such as these provide the necessary markers to delineate the mechanism and biological consequences of transcriptional repression mediated by p53.

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Acknowledgements

We would like to thank Suzanne Cassel for excellent assistance during the early phase of these studies, and Dr Michael Malim (University of Pennsylvania) for kindly providing the MT-2 and CEM-SS cell lines. This work was supported by grants NIH CA66741 (DL George) and NIH CA41086 AJ Levine, and by a generous grant from the WW Smith Charitable Trust (M Murphy).

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  1. Arnold J Levine

    Present address: The Rockefeller University, 1230 York Avenue, New York, NY, 10021-6399, USA

Authors and Affiliations

  1. >Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, 19104-6069, Pennyslvania, PA, USA

    Jaimo Ahn, Stephen Kratowicz & Donna L George

  2. Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, 19111, Pennyslvania, PA, USA

    Maureen Murphy

  3. Department of Molecular Biology, Princeton University, Princeton, 08544, New Jersey, NJ, USA

    Alan Wang & Arnold J Levine

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  1. Jaimo Ahn
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Ahn, J., Murphy, M., Kratowicz, S. et al. Down-regulation of the stathmin/Op18 and FKBP25 genes following p53 induction. Oncogene 18, 5954–5958 (1999). https://doi.org/10.1038/sj.onc.1202986

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