Abstract
Cdc25 activates maturation promoting factor (MPF) and promotes mitosis by removing the inhibitory phosphate from the Tyr-15 of Cdc2 in human cells. In this study, we searched the interacting protein(s) of human Cdc25C using the yeast two-hybrid screen and identified proliferating cell nuclear antigen (PCNA) as an interacting partner of Cdc25C. The interaction between Cdc25C and PCNA was confirmed in vitro and in vivo. Co-immunoprecipitation analyses using human T cell line, Jurkat, further revealed that Cdc25C interacted with PCNA transiently when cells began to enter mitosis. Immunofluorescence analysis also showed that Cdc25C and PCNA were transiently co-localized in the nucleus at the beginning of M phase. Together with the previous observations of the interaction between various cdc/cyclin and PCNA, our findings strongly suggested a potential role of PCNA at the G2 to M phase transition of cell cycle.
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Abbreviations
- MPF:
-
maturation promoting factor
- PCNA:
-
proliferating cell nuclear antigen
- pol δ:
-
DNA polemerase δ
- M:
-
mitosis
- IPTG:
-
isopropyl β-D-thiogalactopyranoside; PI, propidium iodide
- GST:
-
glutathione-S-transferase
- OA:
-
okadaic acid
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Acknowledgements
We thank Drs P Russell, H Piwnica-Worms and M Nakanishi for the kind gifts of PBSK1, anti-Cdc25C antibody and pGEX-PCNA. This work was supported by Grant-in-aid for exploratory research, a part of Grants from the Ministry of Health, Labour and Welfare, the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and Aichi Cancer Research Foundation.
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Kawabe, T., Suganuma, M., Ando, T. et al. Cdc25C interacts with PCNA at G2/M transition. Oncogene 21, 1717–1726 (2002). https://doi.org/10.1038/sj.onc.1205229
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DOI: https://doi.org/10.1038/sj.onc.1205229
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