Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats

Yasumasa Okazaki; Isato Kono; Takayoshi Kuriki; Satomi Funahashi; Soichiro Fushimi; Mohammad Iqbal; Shigeru Okada; Shinya Toyokuni

doi:10.3164/jcbn.11-100

Original Articles

Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats

Yasumasa Okazaki, Isato Kono, Takayoshi Kuriki, Satomi Funahashi, Soichiro Fushimi, Mohammad Iqbal, Shigeru Okada, Shinya Toyokuni

Author information

Yasumasa Okazaki
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Isato Kono
Industrial Technology Center of Okayama Prefecture
Takayoshi Kuriki
Okayama Prefectual Center for Animal Husbandry & Research
Satomi Funahashi
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Soichiro Fushimi
Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Mohammad Iqbal
Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Biotechnology Research Institute, University Malaysia Sabah
Shigeru Okada
Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Department of Anti-Aging Food Sciences, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Shinya Toyokuni
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine

Keywords: lactoferrin, chemoprevention, ferric nitrilotriacetate, oxidative renal damage, glutathione metabolism

JOURNAL FREE ACCESS

2012 Volume 51 Issue 2 Pages 84-90

DOI https://doi.org/10.3164/jcbn.11-100

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Abstract

Milk provides a well-balanced source of amino acids and other ingredients. One of the functional ingredients in milk is lactoferrin (LF). LF presents a wide variety of bioactivities and functions as a radical scavenger in models using iron-ascorbate complexes and asbestos. Human clinical trials of oral LF administration for the prevention of colon polyps have been successful and demonstrated that dietary compounds exhibit direct interactions. However, antioxidative properties of LF in distant organs require further investigation. To study the antioxidant property of LF, we employed bovine lactoferrin (bLF) using the rat model of ferric nitrilotriacetate (Fe-NTA)-induced renal tubular oxidative injury. We fed rats with bLF (0.05%, w/w) in basal chow for 4 weeks and sacrificed them after Fe-NTA treatment. After intraperitoneal administration of 9.0 mg iron/kg Fe-NTA for 4 and 24 h, bLF pretreatment suppressed elevation of serum creatinine and blood urea nitrogen levels. In addition, we observed protective effects against renal oxidative tubular damage and maintenance of antioxidant enzyme activities in the bLF-pretreated group. We thus demonstrated the antioxidative effect of bLF against Fe-NTA-induced renal oxidative injury. These results suggest that LF intake is useful for the prevention of renal tubular oxidative damage mediated by iron.

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