Abstract
P-glycoprotein (P-gp), encoded by the MDR1 gene, is a plasma membrane transporter which confers resistance to many chemotherapeutic drugs. Monoclonal antibodies raised against P-gp have been used as tools to study P-gp topology and activity. Monoclonal antibody UIC2 recognizes a functional conformation of P-gp on the cell surface and blocks P-gp-mediated drug transport. Knowledge about the UIC2 epitope and the mechanism of its inhibitory effects may be helpful for understanding P-gp structure and developing P-gp inhibitors. In the present work, using several chimeras of MDR1 and MDR2, we found that the native sequence of the predicted extracellular loop between transmembrane domains (TM) 5 and 6 of P-gp is necessary, but not sufficient, for UIC2 reactivity. In addition, UIC2 reactivity is also affected by mutations in TM6, a region known to be involved in interactions of P-gp with substrates. These observations suggest that residues in the extracellular loop between TM5 and TM6 are directly involved in the display of the UIC2 epitope. Since TM6 has been shown to be actively involved in drug transport process, the proximity of this region to TM6 may help to explain why UIC2 binding is sensitive to the functional state of P-gp and why binding of UIC2 inhibits P-gp-mediated drug transport.
Copyright 1999 Academic Press.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B / chemistry
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ATP Binding Cassette Transporter, Subfamily B / genetics
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ATP Binding Cassette Transporter, Subfamily B / metabolism
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / chemistry
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / immunology*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
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ATP-Binding Cassette Transporters / chemistry
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism
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Amino Acid Sequence
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Amino Acid Substitution
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / pharmacology
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Antibody Specificity
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Binding Sites / drug effects
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Biological Transport / drug effects
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Cyclosporine / pharmacology
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Epitopes / chemistry
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Epitopes / genetics
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Epitopes / immunology*
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Flow Cytometry
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Fluorescent Dyes / metabolism
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HeLa Cells
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Humans
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Molecular Sequence Data
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Mutation
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Protein Conformation / drug effects
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Transfection
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Vinblastine / pharmacology
Substances
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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ATP-Binding Cassette Transporters
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Antibodies, Monoclonal
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Epitopes
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Fluorescent Dyes
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Recombinant Fusion Proteins
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Vinblastine
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Cyclosporine
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multidrug resistance protein 3