alpha(1)-adrenoceptor-induced trafficking of aquaporin-5 to the apical plasma membrane of rat parotid cells

Biochem Biophys Res Commun. 1999 Nov;265(1):94-100. doi: 10.1006/bbrc.1999.1630.

Abstract

Incubation of rat parotid tissue with 10 microM epinephrine resulted in a transient and marked trafficking of aquaporin-5 (AQP5) from intracellular membranes to the apical plasma membrane (APM) that was maximal at 1 min. This effect of epinephrine was mimicked by phenylephrine, but not by clonidine, dobutamine, or salbutamol, and it was inhibited by phentolamine, but not by propranolol. Furthermore, the epinephrine-induced trafficking of AQP5 was inhibited by phospholipase C inhibitor U73122 as well as dantrolene and TMB-8, both of which inhibit the release of Ca(2+) from intracellular stores. Cytochalasin D and tubulozole-C also inhibited this action of epinephrine. These results indicate that epinephrine, acting at alpha(1)-adrenoceptors, induces the trafficking of AQP5 to the APM by triggering the release of Ca(2+) from intracellular stores through inositol 1,4,5-trisphosphate and ryanodine receptors. In addition, the potent involvement of the cytoskeleton was shown in the epinephrine-induced trafficking of AQP5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Agonists / pharmacology
  • Adrenergic alpha-Antagonists / pharmacology
  • Animals
  • Aquaporin 5
  • Aquaporins / metabolism*
  • Calcium / metabolism
  • Calcium Channels / drug effects
  • Calcium Channels / physiology
  • Cell Membrane / drug effects
  • Cell Membrane / physiology*
  • Epinephrine / pharmacology
  • Estrenes / pharmacology
  • Inositol 1,4,5-Trisphosphate Receptors
  • Kinetics
  • Male
  • Membrane Proteins*
  • Parotid Gland / drug effects
  • Parotid Gland / physiology*
  • Phentolamine / pharmacology
  • Phenylephrine / pharmacology
  • Pyrrolidinones / pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Adrenergic, alpha-1 / physiology*
  • Receptors, Cytoplasmic and Nuclear / drug effects
  • Receptors, Cytoplasmic and Nuclear / physiology
  • Ryanodine Receptor Calcium Release Channel / drug effects
  • Ryanodine Receptor Calcium Release Channel / physiology
  • Type C Phospholipases / antagonists & inhibitors

Substances

  • Adrenergic alpha-Agonists
  • Adrenergic alpha-Antagonists
  • Aqp5 protein, rat
  • Aquaporin 5
  • Aquaporins
  • Calcium Channels
  • Estrenes
  • Inositol 1,4,5-Trisphosphate Receptors
  • Membrane Proteins
  • Pyrrolidinones
  • Receptors, Adrenergic, alpha-1
  • Receptors, Cytoplasmic and Nuclear
  • Ryanodine Receptor Calcium Release Channel
  • 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
  • Phenylephrine
  • Type C Phospholipases
  • Calcium
  • Epinephrine
  • Phentolamine