Abstract
Incubation of rat parotid tissue with 10 microM epinephrine resulted in a transient and marked trafficking of aquaporin-5 (AQP5) from intracellular membranes to the apical plasma membrane (APM) that was maximal at 1 min. This effect of epinephrine was mimicked by phenylephrine, but not by clonidine, dobutamine, or salbutamol, and it was inhibited by phentolamine, but not by propranolol. Furthermore, the epinephrine-induced trafficking of AQP5 was inhibited by phospholipase C inhibitor U73122 as well as dantrolene and TMB-8, both of which inhibit the release of Ca(2+) from intracellular stores. Cytochalasin D and tubulozole-C also inhibited this action of epinephrine. These results indicate that epinephrine, acting at alpha(1)-adrenoceptors, induces the trafficking of AQP5 to the APM by triggering the release of Ca(2+) from intracellular stores through inositol 1,4,5-trisphosphate and ryanodine receptors. In addition, the potent involvement of the cytoskeleton was shown in the epinephrine-induced trafficking of AQP5.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic alpha-Agonists / pharmacology
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Adrenergic alpha-Antagonists / pharmacology
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Animals
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Aquaporin 5
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Aquaporins / metabolism*
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Calcium / metabolism
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Calcium Channels / drug effects
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Calcium Channels / physiology
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Cell Membrane / drug effects
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Cell Membrane / physiology*
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Epinephrine / pharmacology
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Estrenes / pharmacology
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Inositol 1,4,5-Trisphosphate Receptors
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Kinetics
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Male
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Membrane Proteins*
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Parotid Gland / drug effects
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Parotid Gland / physiology*
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Phentolamine / pharmacology
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Phenylephrine / pharmacology
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Pyrrolidinones / pharmacology
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Rats
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Rats, Wistar
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Receptors, Adrenergic, alpha-1 / physiology*
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Receptors, Cytoplasmic and Nuclear / drug effects
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Receptors, Cytoplasmic and Nuclear / physiology
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Ryanodine Receptor Calcium Release Channel / drug effects
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Ryanodine Receptor Calcium Release Channel / physiology
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Type C Phospholipases / antagonists & inhibitors
Substances
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Adrenergic alpha-Agonists
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Adrenergic alpha-Antagonists
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Aqp5 protein, rat
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Aquaporin 5
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Aquaporins
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Calcium Channels
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Estrenes
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Inositol 1,4,5-Trisphosphate Receptors
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Membrane Proteins
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Pyrrolidinones
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Receptors, Adrenergic, alpha-1
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Receptors, Cytoplasmic and Nuclear
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Ryanodine Receptor Calcium Release Channel
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1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
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Phenylephrine
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Type C Phospholipases
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Calcium
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Epinephrine
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Phentolamine