Abstract
Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin and successfully induced the rhythmic expression of Per1, Per2 and dbp. In the initial step of the circadian gene expression, a marked transient induction of Per1 was observed accompanied with CREB phosphorylation. Thus the present study strongly suggests that CREB activation through the cAMP/PKA pathway is involved in the generation of circadian rhythm in rat-1 cells
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activating Transcription Factor 2
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Animals
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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Cell Cycle Proteins
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Cells, Cultured
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Circadian Rhythm / genetics
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Colforsin / pharmacology*
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Cyclic AMP Response Element-Binding Protein / metabolism
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Dicarboxylic Acid Transporters
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Gene Expression
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / genetics*
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Period Circadian Proteins
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Phosphorylation
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Rats
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Transcription Factors / metabolism
Substances
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Activating Transcription Factor 2
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Carrier Proteins
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Cell Cycle Proteins
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Cyclic AMP Response Element-Binding Protein
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Dicarboxylic Acid Transporters
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Nuclear Proteins
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Per1 protein, mouse
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Per1 protein, rat
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Per2 protein, mouse
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Per2 protein, rat
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Period Circadian Proteins
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Transcription Factors
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Colforsin