Abstract
Mitogen-activated protein kinase phosphatase 1 (MKP-1) is negatively regulating mitogen-activated protein kinases and is therefore involved in early signaling processes. The expression of the mkp-1 gene is induced by growth factors and stress. The promoters of the human and murine mkp-1 genes contain several conserved DNA binding elements, including two cAMP response elements and an E box. We observed that the upstream stimulatory factor (USF), but not c-Myc, activated mkp-1. USF synergized with protein kinase A, thus providing evidence for a role of the E box, during signal-regulated stimulation of mkp-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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Basic-Leucine Zipper Transcription Factors
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COS Cells
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Cell Cycle Proteins*
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Colforsin / pharmacology
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Cyclic AMP-Dependent Protein Kinases / genetics
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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DNA / genetics
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DNA / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Dimerization
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Dual Specificity Phosphatase 1
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Enzyme Activation / drug effects
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Genes, Reporter / genetics
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Humans
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Immediate-Early Proteins / genetics*
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Mice
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Molecular Sequence Data
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Phosphoprotein Phosphatases*
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Promoter Regions, Genetic / genetics
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Protein Binding
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Protein Phosphatase 1
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Protein Tyrosine Phosphatases / genetics*
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Proto-Oncogene Proteins c-myc / genetics
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Proto-Oncogene Proteins c-myc / metabolism*
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Response Elements / genetics
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Sequence Alignment
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Signal Transduction* / drug effects
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation* / drug effects
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Upstream Stimulatory Factors
Substances
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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Basic-Leucine Zipper Transcription Factors
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Cell Cycle Proteins
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DNA-Binding Proteins
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Immediate-Early Proteins
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MAX protein, human
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Myc associated factor X
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Proto-Oncogene Proteins c-myc
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Transcription Factors
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USF1 protein, human
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Upstream Stimulatory Factors
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Usf1 protein, mouse
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Max protein, mouse
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Colforsin
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DNA
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Cyclic AMP-Dependent Protein Kinases
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Phosphoprotein Phosphatases
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Protein Phosphatase 1
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DUSP1 protein, human
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Dual Specificity Phosphatase 1
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Dusp1 protein, mouse
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Protein Tyrosine Phosphatases