Synergistic activation of the mkp-1 gene by protein kinase A signaling and USF, but not c-Myc

FEBS Lett. 2000 Jun 2;474(2-3):146-50. doi: 10.1016/s0014-5793(00)01566-0.

Abstract

Mitogen-activated protein kinase phosphatase 1 (MKP-1) is negatively regulating mitogen-activated protein kinases and is therefore involved in early signaling processes. The expression of the mkp-1 gene is induced by growth factors and stress. The promoters of the human and murine mkp-1 genes contain several conserved DNA binding elements, including two cAMP response elements and an E box. We observed that the upstream stimulatory factor (USF), but not c-Myc, activated mkp-1. USF synergized with protein kinase A, thus providing evidence for a role of the E box, during signal-regulated stimulation of mkp-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic-Leucine Zipper Transcription Factors
  • COS Cells
  • Cell Cycle Proteins*
  • Colforsin / pharmacology
  • Cyclic AMP-Dependent Protein Kinases / genetics
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • DNA / genetics
  • DNA / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Dimerization
  • Dual Specificity Phosphatase 1
  • Enzyme Activation / drug effects
  • Genes, Reporter / genetics
  • Humans
  • Immediate-Early Proteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases*
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Protein Phosphatase 1
  • Protein Tyrosine Phosphatases / genetics*
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Response Elements / genetics
  • Sequence Alignment
  • Signal Transduction* / drug effects
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation* / drug effects
  • Upstream Stimulatory Factors

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic-Leucine Zipper Transcription Factors
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Immediate-Early Proteins
  • MAX protein, human
  • Myc associated factor X
  • Proto-Oncogene Proteins c-myc
  • Transcription Factors
  • USF1 protein, human
  • Upstream Stimulatory Factors
  • Usf1 protein, mouse
  • Max protein, mouse
  • Colforsin
  • DNA
  • Cyclic AMP-Dependent Protein Kinases
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • DUSP1 protein, human
  • Dual Specificity Phosphatase 1
  • Dusp1 protein, mouse
  • Protein Tyrosine Phosphatases