Abstract
Intersectin 1 (ITSN1) is a binding partner of dynamin that has been shown to participate in clathrin-mediated endocytosis. Here we report the characterization of a new human gene, ITSN2, highly similar to ITSN1. Alternative splicing of ITSN2 generates a short isoform with two EH domains, a coiled-coil region and five SH3 domains, and a longer isoform containing extra carboxy domains (DH, PH and C2 domains), suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases. ITSN2 expression analysis indicates that it is widely expressed in human tissues. Intersectin 2 isoforms show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin internalization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport*
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Alternative Splicing / genetics
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Amino Acid Sequence
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Animals
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Base Sequence
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COS Cells
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Calcium-Binding Proteins / analysis
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Clathrin / metabolism*
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Cloning, Molecular
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DNA, Complementary / genetics
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Endocytosis*
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Fluorescent Antibody Technique
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Gene Expression Profiling
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Humans
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Phosphoproteins / analysis
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Transfection
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Transferrin / metabolism
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src Homology Domains
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Calcium-Binding Proteins
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Carrier Proteins
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Clathrin
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DNA, Complementary
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EPS15 protein, human
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ITSN2 protein, human
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Intracellular Signaling Peptides and Proteins
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Phosphoproteins
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Protein Isoforms
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RNA, Messenger
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Transferrin
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intersectin 1