Abstract
Mitogen activation of human T-lymphocytes induces a morphine-binding site. Morphine binding is displaceable by beta-endorphin (1--31) and (--)-naloxone but not DAMGO. This site is not stereoselective for (--)-morphine. T-lymphocytes, expressing this binding site, were assayed by reverse-transcription polymerase chain reaction (RT-PCR) for expression of hMOR-1 mRNA. Several primer sets were used and each assay compared with cells known to express human or mouse MOR-1 mRNA. Neither hMOR-1 nor any homologous receptor was detected in human T-lymphocytes. Therefore, the morphine-binding site on mitogen-activated T-lymphocytes is unlikely to be closely related to hMOR-1.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites / physiology
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Binding, Competitive / physiology
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Calcium / metabolism
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DNA Primers / genetics
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DNA Primers / immunology
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DNA Primers / metabolism
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Humans
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Interleukin-2 / pharmacokinetics
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Morphine / immunology
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Morphine / metabolism*
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Narcotics / immunology
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Narcotics / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / immunology
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RNA, Messenger / metabolism
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Receptors, Opioid, mu / genetics
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Receptors, Opioid, mu / immunology
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Receptors, Opioid, mu / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Sodium / metabolism
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism*
Substances
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DNA Primers
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Interleukin-2
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Narcotics
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RNA, Messenger
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Receptors, Opioid, mu
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Morphine
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Sodium
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Calcium