Abstract
1. The adenosine receptor in mouse pinealocytes was identified and characterized using pharmacological and physiological approaches. 2. Expression of the two adenosine receptor subtypes A2B and A3 was detected in mouse pineal glands and PGT-beta cells by polymerase chain reaction and nucleotide sequencing. 3. Adenosine and 5'-N-ethylcarboxamidoadenosine (NECA) evoked cyclic AMP generation but the A2)-selective agonist 2-(4-(2-carboxyethyl)phenylethylamino)adenosine-5'-N-ethylcarboxamideadenosine (CGS 21680) and the A1-specific agonists R-N(6)-(2-phenylisopropyl)adenosine (R-PIA) and N(6)-cyclopentyladenosine (CPA) had little effect on intracellular cyclic AMP levels. The A2B receptor selective antagonists alloxazine and enprofylline completely blocked NECA-mediated cyclic AMP accumulation. 4. Treatment of cells with the A3-selective agonist N(6)-(3-iodobenzyl)-5'-(N-methylcarbamoyl)adenosine (IB-MECA) inhibited the elevation of the cyclic AMP level induced by NECA or isoproterenol in a concentration-dependent manner with maximal inhibition of 40 - 50%. These responses were blocked by the specific A3 adenosine receptor antagonist MRS 1191. Pretreatment of the cells with pertussis toxin attenuated the IB-MECA-induced responses, suggesting that this effect occurred via the pertussis toxin-sensitive inhibitory G proteins. 5. IB-MECA also caused a concentration-dependent elevation in [Ca(2+)]i and IP3 content. Both the responses induced by IB-MECA were attenuated by treatment with U73122 or phorbol 12-myristate 13-acetate. 6. These data suggest the presence of both A2B and A3 adenosine receptors in mouse pineal tumour cells and that the A2B receptor is positively coupled to adenylyl cyclase whereas the A3 receptor is negatively coupled to adenylyl cyclase and also coupled to phospholipase C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / pharmacology
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Adenosine / analogs & derivatives*
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Adenosine / pharmacology
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Adenosine Triphosphate / pharmacology
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Adenosine-5'-(N-ethylcarboxamide) / pharmacology
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Adenylate Cyclase Toxin
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Adenylyl Cyclases / metabolism
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Animals
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Calcium / metabolism
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Colforsin / pharmacology
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Cyclic AMP / metabolism
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Dihydropyridines / pharmacology
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Dose-Response Relationship, Drug
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Enzyme Activation / drug effects
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Estrenes / pharmacology
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GTP-Binding Proteins / drug effects
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GTP-Binding Proteins / metabolism
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Gene Expression Regulation, Neoplastic / drug effects
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Inositol 1,4,5-Trisphosphate / metabolism
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Isoproterenol / pharmacology
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Mice
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Mice, Inbred CBA
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Pertussis Toxin
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Phospholipases / metabolism
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Pinealoma / metabolism*
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Pinealoma / pathology
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Pyrrolidinones / pharmacology
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor, Adenosine A2B
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Receptor, Adenosine A3
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Receptors, Purinergic P1 / drug effects*
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Receptors, Purinergic P1 / genetics
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Receptors, Purinergic P1 / physiology
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Tetradecanoylphorbol Acetate / pharmacology
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Time Factors
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Tumor Cells, Cultured
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Virulence Factors, Bordetella / pharmacology
Substances
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Adenylate Cyclase Toxin
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Dihydropyridines
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Estrenes
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MRS 1191
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Pyrrolidinones
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RNA, Messenger
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Receptor, Adenosine A2B
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Receptor, Adenosine A3
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Receptors, Purinergic P1
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Virulence Factors, Bordetella
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1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
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N(6)-(3-iodobenzyl)-5'-N-methylcarboxamidoadenosine
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Colforsin
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4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
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Adenosine-5'-(N-ethylcarboxamide)
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Inositol 1,4,5-Trisphosphate
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Adenosine Triphosphate
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Cyclic AMP
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Pertussis Toxin
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Phospholipases
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GTP-Binding Proteins
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Adenylyl Cyclases
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Adenosine
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Isoproterenol
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Tetradecanoylphorbol Acetate
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Calcium