Abstract
Rel/NF-kappaB transcription factors control a variety of cellular processes, such as cell growth and apoptosis, that are relevant to oncogenesis, and mutations in genes encoding Rel/NF-kappaB transcription factors have been found in several human lymphoid cell cancers. In this study, we have used a sensitive cell outgrowth assay to demonstrate that wild-type human c-Rel can malignantly transform primary chicken spleen cells, and that transformation by c-Rel is accelerated by co-expression of Bc1-2. Full-length mouse c-Rel can also transform chicken spleen cells. These results are the first demonstration of a lymphoid cell malignant transforming ability for mammalian Rel/NF-kappaB transcription factors, and implicate c-Rel as a molecular target for cancer therapeutics.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Agar
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Animals
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Apoptosis / genetics
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Cell Line, Transformed
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Cell Transformation, Neoplastic / genetics*
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Chickens
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Colony-Forming Units Assay / methods
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Culture Media
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Gene Expression Regulation, Neoplastic
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Genes, bcl-2
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Genes, rel
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Humans
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Mice
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NF-kappa B / physiology
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Oncogene Proteins v-rel / physiology
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / physiology
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Proto-Oncogene Proteins c-rel / genetics
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Proto-Oncogene Proteins c-rel / physiology*
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Recombinant Fusion Proteins / physiology
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Signal Transduction
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Species Specificity
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Spleen / cytology*
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Transcription, Genetic
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Transfection
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bcl-X Protein
Substances
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BCL2L1 protein, human
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Bcl2l1 protein, mouse
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Culture Media
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NF-kappa B
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Oncogene Proteins v-rel
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Proto-Oncogene Proteins c-bcl-2
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Proto-Oncogene Proteins c-rel
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Recombinant Fusion Proteins
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bcl-X Protein
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Agar