Abstract
Histamine, a neurotransmitter and neuroregulatory compound in diverse species, serves as the neurotransmitter of photoreceptors in insects and other arthropods by directly activating a chloride channel. By systematic expression screening of novel putative ligand-gated anion channels, we identified two cDNAs (DM-HisCl-alpha 1 and-alpha 2) coding for putative histamine-gated chloride channels by functional expression in Xenopus laevis oocytes. DM-HisCl-alpha 1 mRNA localizes in the lamina region of the Drosophila eye, supporting the idea that DM-HisCl-alpha 1 may be a neurotransmitter receptor for histamine in the visual system.
MeSH terms
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Animals
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Chloride Channels / genetics*
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Chloride Channels / metabolism
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Cimetidine / pharmacology
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Cloning, Molecular
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DNA, Complementary
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / physiology
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Histamine / metabolism*
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Histamine / pharmacology
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Histamine H2 Antagonists / pharmacology
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In Situ Hybridization
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Ion Channel Gating
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Molecular Sequence Data
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Oocytes / drug effects
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Oocytes / physiology
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Patch-Clamp Techniques
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Reverse Transcriptase Polymerase Chain Reaction
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Xenopus laevis
Substances
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Chloride Channels
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DNA, Complementary
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Drosophila Proteins
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HisCl1 protein, Drosophila
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Histamine H2 Antagonists
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ort protein, Drosophila
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Cimetidine
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Histamine
Associated data
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GENBANK/AF435469
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GENBANK/AF435470
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GENBANK/AF435471