Abstract
We previously identified lynx1 as a neuronal membrane molecule related to snake alpha-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) nAChRs and that lynx1 enhances desensitization. Macroscopic recordings quantify the enhancement of desensitization onset by lynx1 and further show that it slows recovery from desensitization and increases the EC(50). These experiments establish that direct interaction of lynx1 with nAChRs can result in a novel type of functional modulation and suggest that prototoxins may play important roles in vivo by modulating functional properties of their cognate CNS receptors.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylcholine / pharmacology
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Adaptor Proteins, Signal Transducing
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Animals
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Cell Line
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GPI-Linked Proteins
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Humans
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Immunohistochemistry
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism*
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Neurons / cytology
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Neurons / drug effects
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Neurons / metabolism*
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Neuropeptides / chemistry
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Neuropeptides / genetics
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Neuropeptides / metabolism*
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Oocytes / drug effects
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Oocytes / physiology
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Patch-Clamp Techniques
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Protein Structure, Tertiary
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Protein Subunits
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Receptors, GABA-A / genetics
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Receptors, GABA-A / metabolism
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Receptors, Nicotinic / genetics
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Receptors, Nicotinic / metabolism*
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Transfection
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Vasodilator Agents
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Xenopus laevis / physiology
Substances
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Adaptor Proteins, Signal Transducing
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GPI-Linked Proteins
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LYNX1 protein, human
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Membrane Glycoproteins
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Neuropeptides
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Protein Subunits
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Receptors, GABA-A
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Receptors, Nicotinic
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Vasodilator Agents
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Acetylcholine