Activated microglia (BV-2) facilitation of TNF-alpha-mediated motor neuron death in vitro

J Neuroimmunol. 2002 Jul;128(1-2):31-8. doi: 10.1016/s0165-5728(02)00141-8.

Abstract

We have studied the interactions between activated microglia and injured motor neurons using an immortalized murine microglial cell line (BV-2) stimulated with either lipopolysaccharide (LPS) (Escherichia coli) or supernatant from serum-deprived motor neurons (NSC-34 cell line). Both stimuli induced BV-2 activation. Although both BV-2 supernatants induced a subsequent increase in NO generation in otherwise healthy NSC-34 cells, only LPS-activated microglial supernatant induced NSC-34 cell death through a TNF-alpha-dependent pathway. However, we observed a 20-fold increase in the amount of TNF-alpha required to kill NSC-34 cells in the absence of LPS-activated BV-2 cell supernatant, indicating that microglia secrete factor(s) that facilitate TNF-alpha-mediated motor neuron death in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyotrophic Lateral Sclerosis / immunology
  • Amyotrophic Lateral Sclerosis / metabolism
  • Amyotrophic Lateral Sclerosis / physiopathology
  • Animals
  • Antibodies / pharmacology
  • Cell Communication / immunology*
  • Cell Death / immunology*
  • Cell Line, Transformed
  • Cell Movement / drug effects
  • Cell Movement / immunology
  • Cell Survival / drug effects
  • Cell Survival / immunology
  • Central Nervous System / immunology*
  • Central Nervous System / metabolism
  • Central Nervous System / physiopathology
  • Culture Media, Conditioned / pharmacology
  • Culture Media, Serum-Free / pharmacology
  • Gliosis / immunology*
  • Gliosis / metabolism
  • Gliosis / physiopathology
  • Lipopolysaccharides / pharmacology
  • Mice
  • Microglia / immunology*
  • Microglia / metabolism
  • Motor Neurons / immunology*
  • Motor Neurons / metabolism
  • Motor Neurons / pathology
  • Nitric Oxide / biosynthesis
  • Nitric Oxide Synthase / metabolism
  • Phagocytosis / drug effects
  • Phagocytosis / immunology
  • Tumor Necrosis Factor-alpha / immunology*
  • Tumor Necrosis Factor-alpha / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antibodies
  • Culture Media, Conditioned
  • Culture Media, Serum-Free
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Nitric Oxide Synthase