Abstract
To assess the role of orexin receptor signaling in neuron-like cells, Neuro-2a murine neuroblastoma and PC12 human pheochromocytoma cells were stably transfected with human OX(1) or OX(2) receptors. Activation of both receptors strongly elevated cellular inositol phosphates and Ca(2+). A difference in the potency between orexin-A and -B was seen for OX(1), but not OX(2) receptors. Dependence of the orexin-mediated Ca(2+) response on extracellular Ca(2+) and the observed Ba(2+) influx indicate that in addition to phospholipase C, orexin receptors also may couple to similar non-voltage-gated Ca(2+) channels in neuronal cells as previously characterized in non-neuronal cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / physiology
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Carrier Proteins / physiology*
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Humans
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Inositol Phosphates / metabolism
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Intracellular Signaling Peptides and Proteins*
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Neuroblastoma
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Neurons
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Neuropeptides / physiology*
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Neurotransmitter Agents / physiology
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Orexin Receptors
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Orexins
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PC12 Cells
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Pheochromocytoma
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Rats
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide / physiology*
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Recombinant Proteins / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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Carrier Proteins
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HCRT protein, human
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Inositol Phosphates
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Intracellular Signaling Peptides and Proteins
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Neuropeptides
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Neurotransmitter Agents
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Orexin Receptors
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Orexins
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide
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Recombinant Proteins
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Calcium