Abstract
Many components that are derived from medicinal or dietary plants possess potential chemopreventive properties. Curcumin, a yellow coloring agent from turmeric (Curcuma longa Linn, Zingiberaceae), possesses strong antimutagenic and anticarcinogenic activities. In this study, we have found that curcumin inhibits the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor kB (NF-kappaB) activation by preventing the degradation of the inhibitory protein IkBalpa; and the subsequent translocation of the p65 subunit in cultured human promyelocytic leukemia (HL-60) cells. Alternatively, curcumin repressed the TPA-induced activation of NF-kappaB through direct interruption of the binding of NF-kappaB to its consensus DNA sequences. Likewise, the TPA-induced DNA binding of the activator protein-1 (AP-1) was inhibited by curcumin pretreatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anticarcinogenic Agents / pharmacology*
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Carcinogens / pharmacology*
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Consensus Sequence
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Curcumin / pharmacology*
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DNA-Binding Proteins / metabolism
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Electrophoretic Mobility Shift Assay
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Genes, Regulator / physiology
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HL-60 Cells / drug effects
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HL-60 Cells / metabolism
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Humans
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I-kappa B Proteins*
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Leukemia, Myeloid / drug therapy
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Leukemia, Myeloid / metabolism
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NF-KappaB Inhibitor alpha
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NF-kappa B / antagonists & inhibitors*
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Promoter Regions, Genetic
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Protein Transport / drug effects
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Tetradecanoylphorbol Acetate / pharmacology*
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Transcription Factor AP-1 / antagonists & inhibitors*
Substances
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Anticarcinogenic Agents
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Carcinogens
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DNA-Binding Proteins
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I-kappa B Proteins
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NF-kappa B
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NFKBIA protein, human
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Transcription Factor AP-1
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NF-KappaB Inhibitor alpha
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Curcumin
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Tetradecanoylphorbol Acetate