Abstract
Comparisons of the site specific binding of nitrobenzylthioinosine (NBMPR) to intact and lysed red cells from various mammalian and avian species suggest the presence of a cytoplasmic pool of nucleoside transporters. In some species the cytoplasmic pool is about 50% of the total (mouse). On the average, the cytoplasmic pool is approx. 20% of the surface pool of NBMPR-binding sites. In sheep reticulocytes, both pools disappear in an energy-dependent manner during the maturation of the reticulocyte in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Chloromercuribenzenesulfonate / pharmacology
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Affinity Labels
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Animals
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Binding Sites
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Blood Proteins / metabolism
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Carrier Proteins / metabolism*
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Cells, Cultured
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Chick Embryo
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Erythrocyte Membrane / metabolism
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Membrane Proteins / metabolism*
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NADH Dehydrogenase / metabolism
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Nucleoside Transport Proteins
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Reticulocytes / metabolism*
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Sheep
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Thioinosine / analogs & derivatives
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Thioinosine / metabolism
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Trypsin / metabolism
Substances
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Affinity Labels
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Blood Proteins
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Carrier Proteins
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Membrane Proteins
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Nucleoside Transport Proteins
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Thioinosine
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4-Chloromercuribenzenesulfonate
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NADH Dehydrogenase
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Trypsin
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4-nitrobenzylthioinosine