Abstract
We have identified an activator of Rac, P-REX2, that is structurally related to the exchange factor PtdIns(3,4,5)-dependent Rac exchanger (P-REX1), but exhibits distinct tissue-specific expression. P-REX2 is spliced into two RNA species, approximately 3.5 and approximately 10 kb in size. The cDNA corresponding to the smaller transcript encodes a protein that exhibits strong similarity with P-REX1 within its N-terminal domains, but differs in the C-terminal region. P-REX2 promoted increased levels of GTP-bound Rac that could be further stimulated by enhancing PI-3K activity. Thus, P-REX2 may serve as a novel link between Rac activation and the PI-3 kinase pathway.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cell Line
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Cloning, Molecular
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Computational Biology
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Fetus
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GTPase-Activating Proteins / genetics
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GTPase-Activating Proteins / metabolism*
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Genes, Reporter
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Humans
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Kidney
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Luciferases / genetics
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Phosphatidylinositol 3-Kinases / metabolism*
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Protein Biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Transfection
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rac GTP-Binding Proteins / metabolism*
Substances
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GTPase-Activating Proteins
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Guanine Nucleotide Exchange Factors
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PREX1 protein, human
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PREX2 protein, human
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Recombinant Proteins
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Luciferases
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Phosphatidylinositol 3-Kinases
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rac GTP-Binding Proteins