ATP potentiates the formation of AChR aggregate in the co-culture of NG108-15 cells with C2C12 myotubes

FEBS Lett. 2005 Apr 25;579(11):2469-74. doi: 10.1016/j.febslet.2005.03.054.

Abstract

The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. In the co-cultures, antagonized P2Y(1) receptors showed a reduction in NG108-15 cell-induced AChR aggregation. Parallel to this observation, cultured NG108-15 cell secreted ATP into the conditioned medium in a time-dependent manner. Enhancement of ATP release from the cultured NG108-15 cells by overexpression of active mutants of small GTPases increased the aggregation of AChRs in co-culturing with C2C12 myotubes. In addition, ecto-nucleotidase was revealed in the co-culture, which rapidly degraded the applied ATP. These results support the notion that ATP has a role in directing the formation of post-synaptic apparatus in vertebrate neuromuscular junctions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Adenosine Triphosphate / pharmacology*
  • Animals
  • Cell Line
  • Coculture Techniques
  • Mice
  • Monomeric GTP-Binding Proteins / metabolism
  • Muscle Fibers, Skeletal / cytology*
  • Muscle Fibers, Skeletal / drug effects*
  • Muscle Fibers, Skeletal / metabolism
  • Protein Binding / drug effects
  • Protein Structure, Quaternary / drug effects
  • Rats
  • Receptors, Cholinergic / chemistry*
  • Receptors, Cholinergic / metabolism*
  • Receptors, Purinergic P2 / metabolism
  • Receptors, Purinergic P2Y1

Substances

  • P2ry1 protein, mouse
  • P2ry1 protein, rat
  • Receptors, Cholinergic
  • Receptors, Purinergic P2
  • Receptors, Purinergic P2Y1
  • Adenosine Triphosphate
  • Monomeric GTP-Binding Proteins