Abstract
Polycomb group protein Ezh2, one of the key regulators of development in organisms from flies to mice, exerts its epigenetic function through regulation of histone methylation. Here, we report the existence of the cytosolic Ezh2-containing methyltransferase complex and tie the function of this complex to regulation of actin polymerization in various cell types. Genetic evidence supports the essential role of cytosolic Ezh2 in actin polymerization-dependent processes such as antigen receptor signaling in T cells and PDGF-induced dorsal circular ruffle formation in fibroblasts. Revealed function of Ezh2 points to a broader usage of lysine methylation in regulation of both nuclear and extra-nuclear signaling processes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / metabolism*
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Animals
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Antigen Presentation / physiology
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Carrier Proteins / metabolism
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Cell Cycle Proteins / metabolism
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Cell Differentiation / immunology
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Cell Differentiation / physiology
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Cell Proliferation
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Cytoplasm / metabolism
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DNA-Binding Proteins
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Enhancer of Zeste Homolog 2 Protein
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Histone-Lysine N-Methyltransferase
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Humans
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Jurkat Cells
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Lymphocyte Activation
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Neoplasm Proteins
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Nuclear Proteins
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Platelet-Derived Growth Factor / pharmacology
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Polycomb Repressive Complex 2
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Protein Methyltransferases / metabolism*
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Proteins / genetics
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Proteins / metabolism
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Proteins / physiology*
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-vav
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Receptors, Antigen, T-Cell / agonists
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Repressor Proteins / metabolism
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Signal Transduction / physiology*
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T-Lymphocytes / metabolism
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Thymus Gland / immunology
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Thymus Gland / physiology
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Transcription Factors
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cdc42 GTP-Binding Protein / metabolism
Substances
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Actins
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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EED protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Platelet-Derived Growth Factor
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-vav
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Receptors, Antigen, T-Cell
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Repressor Proteins
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SUZ12 protein, human
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Transcription Factors
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VAV1 protein, human
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Vav1 protein, mouse
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Protein Methyltransferases
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EZH2 protein, human
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Enhancer of Zeste Homolog 2 Protein
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Ezh2 protein, mouse
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Histone-Lysine N-Methyltransferase
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Polycomb Repressive Complex 2
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cdc42 GTP-Binding Protein