Abstract
Whole-cell patch-clamp recordings from cultured rat dorsal root ganglion neurons demonstrated that the P2Y1 receptor agonists adenosine 5'-O-2-thiodiphosphate (ADP-beta-S) and 2-methylthio adenosine 5'-diphosphate (2-MeSADP) inhibit the alpha,beta-methylene adenosine 5'-triphosphate (alpha,beta-meATP)-induced P2X3 receptor-currents. This effect could be antagonized by the wide-spectrum G protein blocker GDP-beta-S and the P2Y(1) receptor antagonist MRS 2179. The P2Y12,13 receptor antagonist AR-C6993MX and pertussis toxin, a blocker of Galphai/o, did not interact with the effect of ADP-beta-S. Hence, the results indicate that ADP-sensitive P2Y1 receptors of rat dorsal root ganglion neurons inhibit ionotropic P2X3 receptors via G protein-activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate / analogs & derivatives
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Adenosine Diphosphate / pharmacology
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Adenosine Monophosphate / analogs & derivatives
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Adenosine Monophosphate / pharmacology
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / pharmacology
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Animals
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Animals, Newborn
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Cells, Cultured
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Dose-Response Relationship, Drug
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Ganglia, Spinal / cytology
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Ganglia, Spinal / drug effects
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Ganglia, Spinal / physiology*
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Guanosine 5'-O-(3-Thiotriphosphate) / analogs & derivatives
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Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
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Male
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Neurons / drug effects
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Neurons / physiology*
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Patch-Clamp Techniques
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Pertussis Toxin / pharmacology
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Purinergic P2 Receptor Agonists
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Purinergic P2 Receptor Antagonists
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Rats
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Rats, Wistar
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Receptors, Purinergic P2 / physiology*
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Receptors, Purinergic P2X3
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Receptors, Purinergic P2Y1
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Thionucleotides / pharmacology
Substances
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N(6)-methyl-2'-deoxyadenosine 3',5'-diphosphate
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P2rx3 protein, rat
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P2ry1 protein, rat
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Purinergic P2 Receptor Agonists
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Purinergic P2 Receptor Antagonists
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Receptors, Purinergic P2
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Receptors, Purinergic P2X3
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Receptors, Purinergic P2Y1
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Thionucleotides
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guanosine 5'-O-(2-thiotriphosphate)
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methylthio-ADP
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adenosine 5'-O-(2-thiodiphosphate)
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Guanosine 5'-O-(3-Thiotriphosphate)
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Adenosine Monophosphate
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Adenosine Diphosphate
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cangrelor
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Adenosine Triphosphate
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Pertussis Toxin
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alpha,beta-methyleneadenosine 5'-triphosphate