Fluorescent sensors for rapid monitoring of intracellular cGMP

Viacheslav O Nikolaev; Stepan Gambaryan; Martin J Lohse

doi:10.1038/nmeth816

Fluorescent sensors for rapid monitoring of intracellular cGMP

Nat Methods. 2006 Jan;3(1):23-5. doi: 10.1038/nmeth816.

Authors

Viacheslav O Nikolaev¹, Stepan Gambaryan, Martin J Lohse

Affiliation

¹ Institute of Pharmacology and Toxicology, Versbacher Str. 9, D-97078 Würzburg, Germany.

PMID: 16369548
DOI: 10.1038/nmeth816

Abstract

Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Cells, Cultured
Cyclic AMP / analysis
Cyclic GMP / analysis*
Cyclic GMP-Dependent Protein Kinase Type I
Cyclic GMP-Dependent Protein Kinases / chemistry*
Cyclic GMP-Dependent Protein Kinases / genetics
Fluorescence Resonance Energy Transfer*
Humans
Phosphoric Diester Hydrolases / chemistry*
Phosphoric Diester Hydrolases / genetics
Protein Structure, Tertiary / genetics
Sequence Deletion

Substances

Cyclic AMP
Cyclic GMP-Dependent Protein Kinase Type I
Cyclic GMP-Dependent Protein Kinases
PRKG1 protein, human
Phosphoric Diester Hydrolases
Cyclic GMP