Parkinsonism-inducing neurotoxin MPP+: uptake and toxicity in nonneuronal COS cells expressing dopamine transporter cDNA

S Kitayama; S Shimada; G R Uhl

doi:10.1002/ana.410320120

Parkinsonism-inducing neurotoxin MPP+: uptake and toxicity in nonneuronal COS cells expressing dopamine transporter cDNA

Ann Neurol. 1992 Jul;32(1):109-11. doi: 10.1002/ana.410320120.

Authors

S Kitayama¹, S Shimada, G R Uhl

Affiliation

¹ Laboratory of Molecular Neurobiology, Addiction Research Center/National Institute on Drug Abuse, Johns Hopkins University School of Medicine, Baltimore, MD. 21224.

PMID: 1642464
DOI: 10.1002/ana.410320120

Abstract

Expression of a cloned dopamine transporter complementary DNA in COS cells allows these primate kidney cells to accumulate the parkinsonism-inducing neurotoxin metabolite MPP+ (1-methyl-4-phenylpyridinium) avidly, and MPP+ toxicity results. By documenting that the dopamine transporter can confer MPP+ sensitivity to nonneural cells, these results highlight the key role that this transporter could play in mechanisms underlying parkinsonism.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

1-Methyl-4-phenylpyridinium / pharmacokinetics*
1-Methyl-4-phenylpyridinium / poisoning
Animals
Carrier Proteins / genetics*
Cell Line
DNA / metabolism*
Dopamine Plasma Membrane Transport Proteins
Kidney / cytology
Kidney / metabolism*
Membrane Glycoproteins*
Membrane Transport Proteins*
Nerve Tissue Proteins / genetics
Neurotoxins / pharmacokinetics
Neurotoxins / poisoning
Parkinson Disease, Secondary / chemically induced*

Substances

Carrier Proteins
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins
Membrane Transport Proteins
Nerve Tissue Proteins
Neurotoxins
DNA
1-Methyl-4-phenylpyridinium