Abstract
The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids / pharmacology*
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Aniline Compounds / chemistry
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Aniline Compounds / pharmacokinetics
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Animals
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Binding, Competitive
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Calcium / chemistry
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Calcium / metabolism
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Cell Line
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Dose-Response Relationship, Drug
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacokinetics
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Gene Expression
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Goldfish / metabolism*
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Humans
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Intracellular Fluid / drug effects
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Intracellular Fluid / metabolism
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Receptors, Odorant / agonists*
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Receptors, Odorant / genetics
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Receptors, Odorant / physiology
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Transfection
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Xanthenes / chemistry
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Xanthenes / pharmacokinetics
Substances
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Amino Acids
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Aniline Compounds
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Fluo 4
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Fluorescent Dyes
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Receptors, Odorant
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Xanthenes
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Calcium