Intravenous administration of mannosylated cationic liposome/NFkappaB decoy complexes effectively prevent LPS-induced cytokine production in a murine liver failure model

FEBS Lett. 2006 Jun 26;580(15):3706-14. doi: 10.1016/j.febslet.2006.05.059.

Abstract

The purpose of this study was to inhibit endotoxin induced cytokines production and liver injury by liver non-parenchymal cell (NPC) selective delivery of nuclear factor kappaB (NFkappaB) decoy using mannosylated cationic liposomes (Man-liposomes). In this study, we examined the distribution, inhibitory effect on cytokines production and ALT/AST of intravenously injected Man-liposome/NFkappaB decoy complex. Man-liposome/[(32)P] NFkappaB decoy complexes mostly accumulated in the liver, preferentially in NPC. In a murine lipopolysaccharide-induced liver failure model, the production of tumor necrosis factor-alpha (TNFalpha), IFNgamma, IL1-beta, ALT and AST were effectively reduced by Man-liposome complexes. However, cationic or galactosylated cationic liposome complexes could not inhibit TNFalpha production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cations / chemistry
  • Cell Nucleus / metabolism
  • Cytokines / biosynthesis*
  • Disease Models, Animal
  • Female
  • Inflammation Mediators / metabolism
  • Injections, Intravenous
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / pharmacology
  • Liposomes / administration & dosage
  • Liposomes / chemistry*
  • Liver / injuries
  • Liver Failure / drug therapy*
  • Liver Failure / metabolism
  • Liver Failure / pathology
  • Mannose / chemistry*
  • Mice
  • NF-kappa B / administration & dosage*
  • NF-kappa B / blood
  • NF-kappa B / pharmacology*
  • NF-kappa B / urine
  • Particle Size

Substances

  • Cations
  • Cytokines
  • Inflammation Mediators
  • Lipopolysaccharides
  • Liposomes
  • NF-kappa B
  • Mannose