Isolation of renal proximal tubular brush-border membranes

Jürg Biber; Bruno Stieger; Gerti Stange; Heini Murer

doi:10.1038/nprot.2007.156

Isolation of renal proximal tubular brush-border membranes

Nat Protoc. 2007;2(6):1356-9. doi: 10.1038/nprot.2007.156.

Authors

Jürg Biber¹, Bruno Stieger, Gerti Stange, Heini Murer

Affiliation

¹ Institute of Physiology, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland. juergbiber@access.uzh.ch

PMID: 17545973
DOI: 10.1038/nprot.2007.156

Abstract

This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chemical Fractionation / methods*
Kidney Tubules, Proximal* / ultrastructure
Membranes* / ultrastructure
Mice
Microvilli* / ultrastructure
Rats