Abstract
G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies, Monoclonal / biosynthesis
-
Antibodies, Monoclonal / immunology*
-
Antigen-Antibody Reactions / immunology
-
Blotting, Western
-
Crystallization / methods
-
Crystallography
-
Epitopes / chemistry
-
Epitopes / immunology
-
Fluorescent Dyes / chemistry
-
Humans
-
Immunoglobulin Fab Fragments / chemistry
-
Immunoglobulin Fab Fragments / immunology
-
Mice
-
Molecular Sequence Data
-
Protein Structure, Tertiary
-
Receptors, Adrenergic, beta-2 / chemistry
-
Receptors, Adrenergic, beta-2 / genetics
-
Receptors, Adrenergic, beta-2 / immunology*
-
Receptors, G-Protein-Coupled / chemistry
-
Receptors, G-Protein-Coupled / genetics
-
Receptors, G-Protein-Coupled / immunology*
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / immunology
-
Rhodamines / chemistry
-
Vaccination
Substances
-
Antibodies, Monoclonal
-
Epitopes
-
Fluorescent Dyes
-
Immunoglobulin Fab Fragments
-
Receptors, Adrenergic, beta-2
-
Receptors, G-Protein-Coupled
-
Recombinant Proteins
-
Rhodamines
-
tetramethylrhodamine