Abstract
In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brain regions, including the retina. There, RanBPM is restricted to the inner plexiform layer where it co-localizes with the mGlu8b isoform and processes of cholinergic amacrine cells expressing mGlu2 receptors. RanBPM interacts with mGlu2 and other group II and group III receptors, except mGlu6. Our data suggest that RanBPM might be associated with mGlu receptors at synaptic sites.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / analysis
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Adaptor Proteins, Signal Transducing / metabolism*
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Amacrine Cells / chemistry
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Animals
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Cell Line
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Cholinergic Fibers / chemistry
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Cytoskeletal Proteins / analysis
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Cytoskeletal Proteins / metabolism*
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Humans
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Mice
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Nuclear Proteins / analysis
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Nuclear Proteins / metabolism*
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Rats
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Receptors, Metabotropic Glutamate / analysis
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Receptors, Metabotropic Glutamate / metabolism*
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Retina / cytology
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Retina / metabolism*
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Retina / physiology
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Synaptic Transmission
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Two-Hybrid System Techniques
Substances
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Adaptor Proteins, Signal Transducing
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Cytoskeletal Proteins
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Nuclear Proteins
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Ran binding protein 9
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Receptors, Metabotropic Glutamate
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metabotropic glutamate receptor 2
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metabotropic glutamate receptor 8