Abstract
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA / genetics
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DNA-Binding Proteins / chemistry
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Humans
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Protein Conformation
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Solubility
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Transcription Factor TFIIB
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / isolation & purification
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Transcription, Genetic
Substances
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DNA-Binding Proteins
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Oligonucleotides
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Transcription Factor TFIIB
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Transcription Factors
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DNA
Associated data
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GENBANK/X57450
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GENBANK/X57451
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GENBANK/X57452
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GENBANK/X57453
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GENBANK/X57454
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GENBANK/X57455
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GENBANK/X57688
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GENBANK/X57689
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GENBANK/X59268
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GENBANK/X59436