Abstract
The K562 cell line (chronic myeloid leukemia), sensitive to chemotherapy (non-MDR), and the Lucena cell line, resistant to chemotherapy (MDR) were investigated. The results suggest that both cell lines possess CD34+CD38- profiles of hematopoietic stem cell markers. The promoter regions of ABCB1, ABCG2 and ABCC1 genes contain binding sites for the Oct-4 transcripton factor, which is also considered a marker of tumor stem cells. Lucena cells showed an over-expression of the ABCB1 gene and a high expression of the Oct-4, ABCG2 and ABCC1 genes as compared to K562 cells.
Copyright 2009 Elsevier Ltd. All rights reserved.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ADP-ribosyl Cyclase 1 / genetics
-
ADP-ribosyl Cyclase 1 / metabolism
-
ATP Binding Cassette Transporter, Subfamily B
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
-
ATP Binding Cassette Transporter, Subfamily G, Member 2
-
ATP-Binding Cassette Transporters / genetics
-
Antigens, CD34 / genetics
-
Antigens, CD34 / metabolism
-
Antigens, Surface / genetics
-
Antigens, Surface / metabolism
-
Binding Sites
-
Biomarkers, Tumor / genetics*
-
Biomarkers, Tumor / metabolism
-
Cell Line, Tumor
-
Drug Resistance, Multiple / genetics*
-
Drug Resistance, Multiple / physiology
-
Drug Resistance, Neoplasm / genetics
-
Gene Expression Regulation, Leukemic
-
Humans
-
K562 Cells
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
-
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
-
Membrane Glycoproteins / genetics
-
Membrane Glycoproteins / metabolism
-
Multidrug Resistance-Associated Proteins / genetics
-
Neoplasm Proteins / genetics
-
Neoplastic Stem Cells / metabolism*
-
Neoplastic Stem Cells / pathology
-
Octamer Transcription Factor-3 / genetics
-
Octamer Transcription Factor-3 / metabolism
-
Promoter Regions, Genetic
Substances
-
ABCB1 protein, human
-
ABCG2 protein, human
-
ATP Binding Cassette Transporter, Subfamily B
-
ATP Binding Cassette Transporter, Subfamily B, Member 1
-
ATP Binding Cassette Transporter, Subfamily G, Member 2
-
ATP-Binding Cassette Transporters
-
Antigens, CD34
-
Antigens, Surface
-
Biomarkers, Tumor
-
Membrane Glycoproteins
-
Multidrug Resistance-Associated Proteins
-
Neoplasm Proteins
-
Octamer Transcription Factor-3
-
CD38 protein, human
-
ADP-ribosyl Cyclase 1
-
multidrug resistance-associated protein 1