Relationships between multidrug resistance (MDR) and stem cell markers in human chronic myeloid leukemia cell lines

Leuk Res. 2010 Jun;34(6):757-62. doi: 10.1016/j.leukres.2009.11.004. Epub 2009 Dec 6.

Abstract

The K562 cell line (chronic myeloid leukemia), sensitive to chemotherapy (non-MDR), and the Lucena cell line, resistant to chemotherapy (MDR) were investigated. The results suggest that both cell lines possess CD34+CD38- profiles of hematopoietic stem cell markers. The promoter regions of ABCB1, ABCG2 and ABCC1 genes contain binding sites for the Oct-4 transcripton factor, which is also considered a marker of tumor stem cells. Lucena cells showed an over-expression of the ABCB1 gene and a high expression of the Oct-4, ABCG2 and ABCC1 genes as compared to K562 cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase 1 / genetics
  • ADP-ribosyl Cyclase 1 / metabolism
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters / genetics
  • Antigens, CD34 / genetics
  • Antigens, CD34 / metabolism
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism
  • Binding Sites
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Drug Resistance, Multiple / genetics*
  • Drug Resistance, Multiple / physiology
  • Drug Resistance, Neoplasm / genetics
  • Gene Expression Regulation, Leukemic
  • Humans
  • K562 Cells
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Multidrug Resistance-Associated Proteins / genetics
  • Neoplasm Proteins / genetics
  • Neoplastic Stem Cells / metabolism*
  • Neoplastic Stem Cells / pathology
  • Octamer Transcription Factor-3 / genetics
  • Octamer Transcription Factor-3 / metabolism
  • Promoter Regions, Genetic

Substances

  • ABCB1 protein, human
  • ABCG2 protein, human
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters
  • Antigens, CD34
  • Antigens, Surface
  • Biomarkers, Tumor
  • Membrane Glycoproteins
  • Multidrug Resistance-Associated Proteins
  • Neoplasm Proteins
  • Octamer Transcription Factor-3
  • CD38 protein, human
  • ADP-ribosyl Cyclase 1
  • multidrug resistance-associated protein 1