Abstract
Adenovirus E1a represses transcription of the collagenase gene via the phorbol ester-responsive element (collTRE). The mechanism involves inhibition of the trans-activating function of the transcription factor AP-1 without reduction of its synthesis and without any apparent change in DNA binding or composition. The ability of E1a to downmodulate AP-1 is a unique property among dominant oncogenes. This repression depends on conserved region 1, one of the transforming domains of E1a, indicating that it is an integral feature of adenovirus transformation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenovirus Early Proteins
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Animals
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Base Sequence
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Cell Line
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DNA-Binding Proteins / metabolism*
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Enzyme Repression
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Gene Expression Regulation, Enzymologic
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HeLa Cells / enzymology
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Humans
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Microbial Collagenase / biosynthesis
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Microbial Collagenase / genetics*
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Molecular Sequence Data
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Oncogene Proteins, Viral / metabolism*
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Plasmids
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Promoter Regions, Genetic
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins c-jun
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Receptors, Glucocorticoid / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
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Transfection
Substances
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Adenovirus Early Proteins
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DNA-Binding Proteins
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Oncogene Proteins, Viral
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Proto-Oncogene Proteins c-jun
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Receptors, Glucocorticoid
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Transcription Factors
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Protein-Tyrosine Kinases
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Microbial Collagenase