In vivo effect of the tetrapeptide, N-acetyl-Ser-Asp-Lys-Pro, on the G1-S transition of rat hepatocytes

M N Lombard; D Sotty; J Wdzieczak-Bakala; M Lenfant

doi:10.1111/j.1365-2184.1990.tb01336.x

In vivo effect of the tetrapeptide, N-acetyl-Ser-Asp-Lys-Pro, on the G1-S transition of rat hepatocytes

Cell Tissue Kinet. 1990 Mar;23(2):99-103. doi: 10.1111/j.1365-2184.1990.tb01336.x.

Authors

M N Lombard¹, D Sotty, J Wdzieczak-Bakala, M Lenfant

Affiliation

¹ Institut Curie, Centre Universitaire, Orsay, France.

PMID: 2317837
DOI: 10.1111/j.1365-2184.1990.tb01336.x

Abstract

The synthetic molecule N-Acetyl-Ser-Asp-Lys-Pro (Ac-SDKP), corresponding to the low molecular weight inhibitory factor preventing in vivo haematopoietic stem cell (CFU-S) entry into DNA synthesis, was tested in two heterologous systems in vivo: adult regenerating rat liver and 10-day-old rat hepatocytes synchronized by an irritating trigger. In both systems, it was shown that doses of 2-8 micrograms kg-1 of tetrapeptide inhibited 50-70% of the hepatocyte G1-S transitions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Cycle / drug effects
Cell Cycle / physiology
DNA / biosynthesis
DNA / drug effects
Female
Hematopoietic Stem Cells / drug effects
Hematopoietic Stem Cells / metabolism
Interphase / drug effects
Liver / cytology*
Liver / drug effects
Liver / physiology
Liver Regeneration / drug effects
Liver Regeneration / physiology
Male
Oligopeptides / pharmacology*
Rats
Rats, Inbred Strains

Substances

Oligopeptides
DNA
goralatide