Phorbol ester-induced release of endothelium-derived relaxing factor

Eur J Pharmacol. 1990 Apr 10;179(1-2):207-10. doi: 10.1016/0014-2999(90)90420-b.

Abstract

The effects of a phorbol ester, 12-deoxyphorbol 13-isobutyrate (DPB), on contraction of isolated vascular smooth muscle of rat aorta were examined. DPB (100 nM-1 microM) induced a concentration-dependent contraction in resting aorta. DPB (3-100 nM) also induced a concentration-dependent relaxation of the norepinephrine-induced contraction. The contractile effect of DPB was potentiated whereas the relaxant effect was inhibited by endothelium removal or by methylene blue, suggesting that lower concentrations of DPB release endothelium-derived relaxing factor to inhibit contraction in rat aorta.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta, Thoracic
  • Carbachol / pharmacology
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / physiology
  • Male
  • Muscle Contraction / drug effects*
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / physiology
  • Nitric Oxide / metabolism*
  • Norepinephrine / pharmacology
  • Phorbol Esters / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Vasoconstriction / drug effects
  • Vasodilation / drug effects

Substances

  • Phorbol Esters
  • 12-deoxyphorbol 13-isobutyrate
  • Nitric Oxide
  • Carbachol
  • Norepinephrine