Abstract
Mass spectral and chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal epoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8,11,14-Eicosatrienoic Acid / analogs & derivatives
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8,11,14-Eicosatrienoic Acid / analysis*
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Cytochrome P-450 CYP2J2
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Cytochrome P-450 Enzyme System / metabolism*
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Fatty Acids, Unsaturated / analysis*
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Humans
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Kidney Cortex / analysis*
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Male
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Mass Spectrometry
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Oxygenases / metabolism*
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Stereoisomerism
Substances
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Fatty Acids, Unsaturated
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11,12-epoxy-5,8,14-eicosatrienoic acid
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8,9-epoxyeicosatrienoic acid
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14,15-epoxy-5,8,11-eicosatrienoic acid
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Cytochrome P-450 Enzyme System
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Oxygenases
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Cytochrome P-450 CYP2J2
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8,11,14-Eicosatrienoic Acid