Until recently, release studies have failed to indicate the existence of autoreceptors on motor nerves. Ignaz Wessler now reports on a refinement of the technique - the measurement of newly synthesized [3H]acetylcholine released from the phrenic nerve - which provides clear evidence in support of release-modulating autoreceptors. Presynaptic nicotinic receptors mediate a positive feedback mechanism, can rapidly be desensitized and appear to differ in their pharmacological profile from the postsynaptic receptors. In addition, inhibitory and facilitatory muscarinic receptors appear to be involved in the presynaptic control of transmitter release from the phrenic nerve.