Intracellular picrotoxin blocks pentobarbital-gated Cl- conductance

Neurosci Res. 1988 Oct;6(1):72-5. doi: 10.1016/0168-0102(88)90007-7.

Abstract

Using the 'inside-out' configuration of frog sensory neurons, we studied the effect of intracellular picrotoxin on the pentobarbital-gated single channel response of Cl- -current (iCl). The pentobarbital-induced iCl showed no voltage-dependency and the single channel conductance (gamma Cl) was 16 +/- 3.1 pS (n = 6). Picrotoxin caused the pentobarbital-gated Cl- channels to react in a flickering pattern and then finally caused them to cease their opening altogether. This inhibitory action of picrotoxin was reversible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlorides / physiology*
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / physiology*
  • In Vitro Techniques
  • Membrane Potentials / drug effects
  • Microinjections
  • Pentobarbital / pharmacology*
  • Picrotoxin / pharmacology*
  • Rana catesbeiana

Substances

  • Chlorides
  • Picrotoxin
  • Pentobarbital