Abstract
The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats. Transient exposure of cells to 5-azacytidine, a cytosine analogue which cannot be methylated at the 5 position, results in the hypomethylation and transcriptional activation of ev-1, as well as the acquisition of at least one nuclease-hypersensitive site within the chromosomal domain of ev-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Azacitidine / pharmacology
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Cell Transformation, Viral*
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Chick Embryo
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Chromatin / drug effects
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Chromatin / ultrastructure*
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DNA / genetics*
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DNA Restriction Enzymes
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Deoxyribonuclease I
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Deoxyribonucleases
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Endonucleases
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Methylation
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Retroviridae / genetics*
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Transcription, Genetic / drug effects
Substances
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Chromatin
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DNA
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Deoxyribonucleases
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Endonucleases
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DNA Restriction Enzymes
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Deoxyribonuclease I
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Azacitidine