Presence of VIP receptors in a human pancreatic adenocarcinoma cell line. Modulation of the cAMP response during cell proliferation

Biochem Biophys Res Commun. 1983 Mar 29;111(3):958-63. doi: 10.1016/0006-291x(83)91393-1.

Abstract

It is known that the human exocrine pancreas responds to secretin stimulation more than does VIP, a structurally related peptide. We looked for the receptors for those polypeptides in a human pancreatic cancer cell line grown in culture and in nude mice. By analysing the cAMP responses and the 125I-VIP binding we found VIP receptors with a KD of 1.5 10(-9) M. Secretin stimulates the adenylate cyclase through the VIP receptor sites with a KD of 1.7. 10(-6) M. We noted also that during cell proliferation in culture there was about a 5 fold increase of the cAMP response to VIP.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Animals
  • Cell Division
  • Cell Line
  • Cyclic AMP / metabolism*
  • Humans
  • Mice
  • Mice, Nude
  • Pancreatic Neoplasms / metabolism*
  • Receptors, Cell Surface / isolation & purification*
  • Receptors, Vasoactive Intestinal Peptide
  • Secretin / pharmacology
  • Vasoactive Intestinal Peptide / pharmacology

Substances

  • Receptors, Cell Surface
  • Receptors, Vasoactive Intestinal Peptide
  • Secretin
  • Vasoactive Intestinal Peptide
  • Cyclic AMP