Abstract
Addition of 15L-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) to human leukocytes led to the formation of a novel series of compounds containing four conjugated double bonds. The yield of tetraenes was increased approx. 100-fold when ionophore A23187 (5 microM) was added simultaneously with 15-HPETE. The structure of the major tetraene was established by physical methods as well as by chemical degradation and found to be 5,6,15L-trihydroxy-7,9,11,13-eicosatetraenoic acid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkenes / blood*
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Arachidonic Acid
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Arachidonic Acids / blood*
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Blood Platelets / metabolism
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Calcimycin / pharmacology
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Chemical Phenomena
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Chemistry
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Humans
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Hydroxyeicosatetraenoic Acids*
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Leukocytes / drug effects
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Leukocytes / metabolism*
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Leukotrienes*
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Lipid Peroxides / blood
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Lipoxins*
Substances
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Alkenes
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Arachidonic Acids
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Hydroxyeicosatetraenoic Acids
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Leukotrienes
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Lipid Peroxides
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Lipoxins
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lipoxin A4
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Arachidonic Acid
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Calcimycin
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15-hydroperoxy-5,8,11,13-eicosatetraenoic acid