Trihydroxytetraenes: a novel series of compounds formed from arachidonic acid in human leukocytes

Biochem Biophys Res Commun. 1984 Feb 14;118(3):943-9. doi: 10.1016/0006-291x(84)91486-4.

Abstract

Addition of 15L-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) to human leukocytes led to the formation of a novel series of compounds containing four conjugated double bonds. The yield of tetraenes was increased approx. 100-fold when ionophore A23187 (5 microM) was added simultaneously with 15-HPETE. The structure of the major tetraene was established by physical methods as well as by chemical degradation and found to be 5,6,15L-trihydroxy-7,9,11,13-eicosatetraenoic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkenes / blood*
  • Arachidonic Acid
  • Arachidonic Acids / blood*
  • Blood Platelets / metabolism
  • Calcimycin / pharmacology
  • Chemical Phenomena
  • Chemistry
  • Humans
  • Hydroxyeicosatetraenoic Acids*
  • Leukocytes / drug effects
  • Leukocytes / metabolism*
  • Leukotrienes*
  • Lipid Peroxides / blood
  • Lipoxins*

Substances

  • Alkenes
  • Arachidonic Acids
  • Hydroxyeicosatetraenoic Acids
  • Leukotrienes
  • Lipid Peroxides
  • Lipoxins
  • lipoxin A4
  • Arachidonic Acid
  • Calcimycin
  • 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid