Abstract
In this study we examined the role of rho p21 in neuropeptide-stimulated tyrosine phosphorylation. Intact Swiss 3T3 cells were treated with the Clostridium botulinum C3 exoenzyme which specifically ADP ribosylates and inactivates rho p21. C3 exoenzyme treatment of cells caused a marked decrease in both bombesin- and endothelin-stimulated tyrosine phosphorylation of multiple proteins, including p125 focal adhesion kinase (FAK) and paxillin. Our results suggest that rho p21 is a component of the signal transduction pathway linking seven transmembrane domain receptors with tyrosine phosphorylation and cytoskeletal events.
MeSH terms
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3T3 Cells / metabolism
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ADP Ribose Transferases / genetics
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ADP Ribose Transferases / pharmacology*
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Adenosine Diphosphate Ribose / metabolism
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Animals
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Base Sequence
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Bombesin / pharmacology*
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Botulinum Toxins*
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Cell Adhesion Molecules / metabolism*
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Cytoskeletal Proteins / metabolism*
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Endothelins / pharmacology*
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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GTP-Binding Proteins / metabolism
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Mice
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Molecular Sequence Data
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Molecular Weight
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Paxillin
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Phosphoproteins / metabolism*
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Phosphorylation
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Phosphotyrosine
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Protein-Tyrosine Kinases / metabolism*
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Recombinant Proteins / pharmacology
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Signal Transduction
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Tyrosine / analogs & derivatives*
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Tyrosine / metabolism
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rho GTP-Binding Proteins
Substances
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Cell Adhesion Molecules
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Cytoskeletal Proteins
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Endothelins
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Paxillin
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Phosphoproteins
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Pxn protein, mouse
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Recombinant Proteins
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Adenosine Diphosphate Ribose
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Phosphotyrosine
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Tyrosine
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ADP Ribose Transferases
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exoenzyme C3, Clostridium botulinum
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Protein-Tyrosine Kinases
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Ptk2 protein, mouse
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Botulinum Toxins
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GTP-Binding Proteins
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rho GTP-Binding Proteins
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Bombesin