Identification of a putative steroidogenic factor-1 response element in the DAX-1 promoter

Biochem Biophys Res Commun. 1995 Sep 14;214(2):576-81. doi: 10.1006/bbrc.1995.2324.

Abstract

The nuclear hormone receptor, DAX-1, is responsible for X-linked adrenal hypoplasia congenita and hypogonadotrophic hypogonadism. We recently cloned the 5' flanking region of the human DAX-1 gene and in this report we describe the identification of a putative steroidogenic factor 1 (SF-1) response element approximately 110 bases upstream of the TATA box. Both DAX-1 and SF-1 are expressed in similar tissues including the adrenal cortex, gonads, hypothalamus, and the pituitary gland. Like DAX-1, SF-1 expression has been shown to be essential for the development of the adrenal cortex. We demonstrate that SF-1 is able to efficiently bind to the putative SF-1 response element found in the DAX-1 promoter in vitro. This suggests that SF-1 may directly regulate the expression of DAX-1 and that these two transcription factors may be components of a cascade required for development of steroidogenic tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cell Nucleus / metabolism
  • Consensus Sequence
  • DAX-1 Orphan Nuclear Receptor
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Receptors, Cytoplasmic and Nuclear / biosynthesis*
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Retinoic Acid / biosynthesis
  • Receptors, Retinoic Acid / genetics*
  • Repressor Proteins*
  • Sequence Homology, Nucleic Acid
  • Steroidogenic Factor 1
  • TATA Box
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • DAX-1 Orphan Nuclear Receptor
  • DNA-Binding Proteins
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • NR0B1 protein, human
  • NR5A1 protein, human
  • Oligodeoxyribonucleotides
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Retinoic Acid
  • Repressor Proteins
  • Steroidogenic Factor 1
  • Transcription Factors

Associated data

  • GENBANK/S79548