Abstract
The nuclear hormone receptor, DAX-1, is responsible for X-linked adrenal hypoplasia congenita and hypogonadotrophic hypogonadism. We recently cloned the 5' flanking region of the human DAX-1 gene and in this report we describe the identification of a putative steroidogenic factor 1 (SF-1) response element approximately 110 bases upstream of the TATA box. Both DAX-1 and SF-1 are expressed in similar tissues including the adrenal cortex, gonads, hypothalamus, and the pituitary gland. Like DAX-1, SF-1 expression has been shown to be essential for the development of the adrenal cortex. We demonstrate that SF-1 is able to efficiently bind to the putative SF-1 response element found in the DAX-1 promoter in vitro. This suggests that SF-1 may directly regulate the expression of DAX-1 and that these two transcription factors may be components of a cascade required for development of steroidogenic tissues.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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Cell Nucleus / metabolism
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Consensus Sequence
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DAX-1 Orphan Nuclear Receptor
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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Fushi Tarazu Transcription Factors
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Homeodomain Proteins
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Humans
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction
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Promoter Regions, Genetic*
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Receptors, Cytoplasmic and Nuclear / biosynthesis*
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism
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Receptors, Retinoic Acid / biosynthesis
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Receptors, Retinoic Acid / genetics*
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Repressor Proteins*
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Sequence Homology, Nucleic Acid
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Steroidogenic Factor 1
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TATA Box
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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DAX-1 Orphan Nuclear Receptor
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DNA-Binding Proteins
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Fushi Tarazu Transcription Factors
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Homeodomain Proteins
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NR0B1 protein, human
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NR5A1 protein, human
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Oligodeoxyribonucleotides
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Receptors, Cytoplasmic and Nuclear
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Receptors, Retinoic Acid
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Repressor Proteins
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Steroidogenic Factor 1
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Transcription Factors