Abstract
A complementary DNA encoding an inward rectifier K+ channel (IRK1) was isolated from a mouse macrophage cell line by expression cloning. This channel conducts inward K+ current below the K+ equilibrium potential but passes little outward K+ current. The IRK1 channel contains only two putative transmembrane segments per subunit and corresponds to the inner core structure of voltage-gated K+ channels. The IRK1 channel and an ATP-regulated K+ channel show extensive sequence similarity and constitute a new superfamily.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Cell Line
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Cloning, Molecular
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DNA / genetics*
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DNA / isolation & purification
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Gene Library
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Guinea Pigs
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Macrophages / physiology*
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Membrane Proteins / genetics*
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Membrane Proteins / physiology
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Mice
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Models, Structural
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Molecular Sequence Data
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Multigene Family*
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Oocytes / physiology
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Poly A / genetics
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Poly A / isolation & purification
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Potassium Channels / genetics*
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Potassium Channels / physiology
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Potassium Channels, Inwardly Rectifying*
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Xenopus
Substances
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Membrane Proteins
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Potassium Channels
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Potassium Channels, Inwardly Rectifying
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RNA, Messenger
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Recombinant Proteins
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Poly A
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RNA
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DNA