Abstract
Syntaxins A and B are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. A family of syntaxin-related proteins from rat has been identified that shares 23%-84% amino acid identity. Each of the six syntaxins terminate with a carboxy-terminal hydrophobic domain that anchors the protein on the cytoplasmic surface of cellular membranes. The syntaxins display a broad tissue distribution and, when expressed in COS cells, are targeted to different subcellular compartments. Microinjection studies suggest that the nervous system-specific syntaxin 1A is important for calcium-regulated secretion from neuro-endocrine PC12 cells. These results indicate that the syntaxins are a family of receptors for intracellular transport vesicles and that each target membrane may be identified by a specific member of the syntaxin family.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antigens, Surface / genetics
-
Antigens, Surface / metabolism*
-
Blotting, Northern
-
Cell Line
-
Cell Membrane / metabolism
-
DNA / genetics
-
DNA / metabolism
-
Gene Library
-
Molecular Sequence Data
-
Nerve Tissue Proteins / genetics
-
Nerve Tissue Proteins / metabolism*
-
PC12 Cells
-
Poly A / analysis
-
Poly A / metabolism
-
Protein Biosynthesis
-
RNA / analysis
-
RNA / metabolism
-
RNA, Messenger
-
Rats
-
Sequence Homology, Amino Acid
-
Synaptic Vesicles / metabolism*
-
Syntaxin 1
-
Transcription, Genetic
-
Transfection
Substances
-
Antigens, Surface
-
Nerve Tissue Proteins
-
RNA, Messenger
-
Stx1a protein, rat
-
Syntaxin 1
-
Poly A
-
RNA
-
DNA
Associated data
-
GENBANK/L20820
-
GENBANK/L20821
-
GENBANK/L20822
-
GENBANK/L20823
-
GENBANK/L20888
-
GENBANK/L20889
-
GENBANK/L33709
-
GENBANK/M95734
-
GENBANK/M95735
-
GENBANK/S60904
-
GENBANK/S60905
-
GENBANK/S60924