Abstract
Addition of phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell-secreted NGF. The effect of PC-PLC was 2.5 times higher than that elicited by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC-PLC was reduced-though still evident-and similar to that exerted by sphingosine. Results thus indicate that PC-PLC induces the synthesis of NGF by glial cells by a PKC-dependent and PKC-independent mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Brain / cytology
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Cells, Cultured
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Enzyme Activation / drug effects
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Hydrolysis
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Nerve Growth Factors / biosynthesis*
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Nerve Growth Factors / genetics
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Neuroglia / metabolism*
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Phosphatidylcholines / metabolism*
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Protein Kinase C / metabolism
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RNA, Messenger / biosynthesis
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Rats
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Sphingosine / pharmacology
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Tetradecanoylphorbol Acetate / pharmacology
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Type C Phospholipases / metabolism
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Type C Phospholipases / pharmacology*
Substances
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Nerve Growth Factors
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Phosphatidylcholines
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RNA, Messenger
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Protein Kinase C
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Type C Phospholipases
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Sphingosine
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Tetradecanoylphorbol Acetate