Abstract
The neuronal cannabinoid receptor clone was expressed of saturable [3H]WIN 55,212-2 binding sites. Co-expression of the cannabinoid receptor with cRNA coding for the G-protein-gated inwardly rectifying K+ channel (GIRK1) resulted in oocytes exhibiting large inward K+ currents in response to the cannabinoid agonist WIN 55,212-2. The activation of the potassium current by WIN 55,212-2 was dose-dependent with an EC50 of 630 nM. These results suggest that activation of inwardly rectifying K+ channels may be an additional effector mechanism for brain cannabinoid receptors.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analgesics / pharmacology
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Animals
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Benzoxazines
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GTP-Binding Proteins / metabolism
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Ion Channel Gating / physiology
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Kinetics
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Morpholines / pharmacology
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Naphthalenes / pharmacology
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Oocytes / metabolism*
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Patch-Clamp Techniques
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Potassium Channels / metabolism*
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RNA, Complementary / biosynthesis
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Rats
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Receptors, Cannabinoid
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Receptors, Drug / agonists
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Receptors, Drug / biosynthesis
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Receptors, Drug / metabolism*
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Xenopus
Substances
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Analgesics
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Benzoxazines
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Morpholines
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Naphthalenes
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Potassium Channels
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RNA, Complementary
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Receptors, Cannabinoid
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Receptors, Drug
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(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone
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GTP-Binding Proteins