Immunodetection of putative imidazoline receptor proteins in the human and rat brain and other tissues

P V Escribá; M Sastre; H Wang; S Regunathan; D J Reis; J A García-Sevilla

doi:10.1016/0304-3940(94)90295-x

Immunodetection of putative imidazoline receptor proteins in the human and rat brain and other tissues

Neurosci Lett. 1994 Aug 29;178(1):81-4. doi: 10.1016/0304-3940(94)90295-x.

Authors

P V Escribá¹, M Sastre, H Wang, S Regunathan, D J Reis, J A García-Sevilla

Affiliation

¹ Department of Fundamental Biology and Health Sciences, University of the Balearic Islands, Palma de Mallorca, Spain.

PMID: 7816347
DOI: 10.1016/0304-3940(94)90295-x

Abstract

A specific anti-imidazoline receptor (IR) protein antiserum was used to detect these putative receptors in brain and other tissues. In immunoblotting experiments, various brain areas and tissues expressed a double band of 29-30 kDa, and some of them bands of 47 and 66 kDa. The human medulla oblongata also showed bands of 44 and 50 kDa; and the rat adrenal medulla, bands of 58 and 86 kDa. Total immunoreactivity correlated with the density of IR binding sites in the various tissues. This is first evidence of heterogeneity of immunoreactive IRs in the mammalian brain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adrenal Medulla / chemistry
Adrenergic alpha-Agonists / metabolism
Adrenergic alpha-Antagonists / metabolism
Animals
Autoradiography
Brain Chemistry*
Chromatography, Affinity
Clonidine / analogs & derivatives
Clonidine / metabolism
Dioxanes / metabolism
Electrophoresis, Polyacrylamide Gel
Humans
Idazoxan
Imidazoline Receptors
Immunoblotting
Liver / chemistry
Male
Medulla Oblongata / chemistry
Rats
Rats, Sprague-Dawley
Receptors, Drug / analysis*
Receptors, Drug / isolation & purification
Receptors, Drug / metabolism
Tritium

Substances

Adrenergic alpha-Agonists
Adrenergic alpha-Antagonists
Dioxanes
Imidazoline Receptors
Receptors, Drug
Tritium
apraclonidine
2-methoxyidazoxan
Clonidine
Idazoxan