Abstract
SH-PTP2, the vertebrate homolog of Drosophila corkscrew, associates with several activated growth factor receptors, but its biological function is unknown. We assayed the effects of injection of wild-type and mutant SH-PTP2 RNAs on Xenopus embryogenesis. An internal phosphatase domain deletion (delta P) acts as a dominant negative mutant, causing severe posterior truncations. This phenotype is rescued by SH-PTP2, but not by the closely related SH-PTP1. In ectodermal explants, delta P blocks fibroblast growth factor (FGF)- and activin-mediated induction of mesoderm and FGF-induced mitogen-activated protein (MAP) kinase activation. Our results indicate that SH-PTP2 is required for early vertebrate development, acting as a positive component in FGF signaling downstream of the FGF receptor and upstream of MAP kinase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activins
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcium-Calmodulin-Dependent Protein Kinases / genetics*
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Calcium-Calmodulin-Dependent Protein Kinases / metabolism
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Cloning, Molecular
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Culture Techniques
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Ectoderm / drug effects
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Ectoderm / physiology*
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Embryonic Induction* / drug effects
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Female
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Fibroblast Growth Factor 2 / pharmacology
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Inhibins / pharmacology
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Intracellular Signaling Peptides and Proteins
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Mesoderm / drug effects
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Mesoderm / physiology*
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Molecular Sequence Data
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases / genetics
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Protein Tyrosine Phosphatases / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / pharmacology
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Sequence Analysis, DNA
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Sequence Deletion / physiology
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Xenopus
Substances
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Intracellular Signaling Peptides and Proteins
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RNA, Messenger
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Fibroblast Growth Factor 2
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Activins
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Inhibins
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Calcium-Calmodulin-Dependent Protein Kinases
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases