Abstract
We report that certain oligonucleotides are capable of inhibiting cell surface induction of the major histocompatibility complex class I (MHC-I) proteins by interferon-gamma in K562 cells. The inhibition by oligodeoxy-nucleotide I 5' GGG GTT GGT TGT GTT GGG TGT TGT GT-RNH2 is dose-dependent, with an EC50 24 hr after dosing of approximately 4 microM for 800 U/ml interferon-gamma. The reverse complement II 5' AC ACA ACA CCC AAC ACA ACC AAC CCC-RNH2 did not show activity. Oligodeoxynucleotide I inhibits induction of MHC-I by interferon-gamma, but does not inhibit induction by either interferon-alpha or interferon-beta. Four other oligodeoxynucleotides were also evaluated, and three showed activity against interferon-gamma at 25 microM.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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Cell Adhesion Molecules / genetics
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Cell Line
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Gene Expression / drug effects
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Genes, MHC Class I*
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Histocompatibility Antigens Class I / genetics
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Histocompatibility Antigens Class I / metabolism*
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Humans
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In Vitro Techniques
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Intercellular Adhesion Molecule-1
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Interferon-gamma / antagonists & inhibitors*
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Molecular Sequence Data
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / pharmacology*
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Receptors, Transferrin / metabolism
Substances
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Cell Adhesion Molecules
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Histocompatibility Antigens Class I
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Transferrin
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Intercellular Adhesion Molecule-1
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Interferon-gamma