Abstract
A neuronal cell line, HT-22, is sensitive to glutamate cytotoxicity via a non-receptor mediated oxidative pathway. 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, blocks this glutamate-induced cell death. Down-regulation of protein kinase C eliminates the protection against glutamate cytotoxicity afforded by TPA. The data suggest that protein kinase C activation blocks an early step in the cytotoxic pathway.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Death / drug effects
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Cell Line
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Cell Survival / drug effects
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Cell Survival / physiology
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Down-Regulation / drug effects
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Enzyme Activation / physiology
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Excitatory Amino Acid Antagonists / pharmacology
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Glutamic Acid / toxicity*
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Hippocampus / cytology
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Hippocampus / metabolism
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Mice
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Neurons / drug effects*
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Neurons / enzymology
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Phosphorylation
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Protein Kinase C / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Excitatory Amino Acid Antagonists
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Glutamic Acid
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Protein Kinase C
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Tetradecanoylphorbol Acetate