Isolation of a cDNA encoding a human rolipram-sensitive cyclic AMP phosphodiesterase (PDE IVD)

Gene. 1994 Jan 28;138(1-2):253-6. doi: 10.1016/0378-1119(94)90818-4.

Abstract

cDNAs encoding human family-IV phosphodiesterase, subtype D (hPDE IVD) were isolated from a human heart cDNA library. The overlapping cDNAs encode a polypeptide of 604 amino acids (aa) with a predicted M(r) of 68,502, which is 91.4% identical to the rat homolog, rPDE IVD. hPDE IVD produced in Escherichia coli was inhibited by rolipram. Expression of the hPDE IVD mRNA is widespread in human tissues and most abundant in skeletal muscle.

Publication types

  • Comparative Study

MeSH terms

  • 3',5'-Cyclic-AMP Phosphodiesterases / biosynthesis*
  • 3',5'-Cyclic-AMP Phosphodiesterases / genetics
  • 3',5'-Cyclic-AMP Phosphodiesterases / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Escherichia coli
  • Female
  • Gene Expression
  • Gene Library
  • Humans
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Muscles / enzymology*
  • Myocardium / enzymology*
  • Organ Specificity
  • Phosphodiesterase Inhibitors / pharmacology*
  • Polymerase Chain Reaction
  • Pregnancy
  • Pyrrolidinones / pharmacology*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / metabolism
  • Rats
  • Rolipram
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • Isoenzymes
  • Phosphodiesterase Inhibitors
  • Pyrrolidinones
  • RNA, Messenger
  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Rolipram

Associated data

  • GENBANK/U02882