Abstract
cDNAs encoding human family-IV phosphodiesterase, subtype D (hPDE IVD) were isolated from a human heart cDNA library. The overlapping cDNAs encode a polypeptide of 604 amino acids (aa) with a predicted M(r) of 68,502, which is 91.4% identical to the rat homolog, rPDE IVD. hPDE IVD produced in Escherichia coli was inhibited by rolipram. Expression of the hPDE IVD mRNA is widespread in human tissues and most abundant in skeletal muscle.
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / biosynthesis*
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3',5'-Cyclic-AMP Phosphodiesterases / genetics
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3',5'-Cyclic-AMP Phosphodiesterases / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / isolation & purification
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Escherichia coli
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Female
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Gene Expression
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Gene Library
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Humans
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Isoenzymes / biosynthesis
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Isoenzymes / genetics
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Isoenzymes / metabolism
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Molecular Sequence Data
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Multigene Family
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Muscles / enzymology*
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Myocardium / enzymology*
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Organ Specificity
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Phosphodiesterase Inhibitors / pharmacology*
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Polymerase Chain Reaction
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Pregnancy
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Pyrrolidinones / pharmacology*
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RNA, Messenger / biosynthesis
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RNA, Messenger / metabolism
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Rats
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Rolipram
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
Substances
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DNA, Complementary
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Isoenzymes
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Phosphodiesterase Inhibitors
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Pyrrolidinones
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RNA, Messenger
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3',5'-Cyclic-AMP Phosphodiesterases
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Rolipram